Institute of Plant Physiological Ecology, Tissuelyser-48: Research on Maize Endosperm Development and Genetic Improvement

 Product Case    |      2017.07.06    |      Institute of Plant Physiological Ecology Tissuelyser-48: Research on Maize Endosperm Development and Genetic Improvement

Maize seed mainly consists of embryo and endosperm, accounting for 10% and 90% of the dry weight respectively, so the normal development of endosperm is a key factor in determining corn yield and quality. Maize seed particles are large, not easy to grind, and the sample is wasted. In turn, the corn endosperm development and genetic improvement research group of the Institute of Plant Physiology and Ecology, Chinese Academy of Sciences purchased the Shanghai Netson automatic sample tissue grinding instrument. Shanghai Netson's automatic tissue rapid grindometer is Grinding in a closed EP tube successfully solves the problem that the manual grinding of the teacher takes time and effort, wasting the sample, and further analyzes the gene expression regulation network for maize endosperm development and the endosperm modification mechanism of quality protein maize (QPM). The gene clones and functional studies of important maize kernel mutants have been facilitated.

There was no significant difference in the concentration and purity of DNA and RNA extracted from LAWSON (Luoshen China) after the automatic sample rapid grindometer processing and manual liquid nitrogen grinding treatment (paired test P>0.05). However, manual grinding has a large sample loss rate. For samples with low DNA extraction concentration, the automatic grinder can achieve sample 0 loss, which has obvious advantages in DNA extraction, and the manual processing process is complicated and easy to cross. Contamination, operation time, etc. are not as good as LAWSON's automatic sample rapid grinding machine, so the automatic grinding machine has great advantages for DNA extraction compared to manual grinding.

LAWSON (Luo Shang China) is currently the only domestic manufacturer of horizontal tissue grinding machines, vertical tissue grinding machines, and three-dimensional integrated tissue grinding machines with a full range of production lines. It is the inventor of multi-sample tissue grinding machines in China and masters core technologies. At the same time also to a number of foreign brands and domestic enterprises to provide OEM, OEM processing services.